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. 2017 Apr 3;7:539. doi: 10.1038/s41598-017-00533-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cryo-EM structure of CPMV-M and CPMV-T. (a) Electron micrographs of CPMV-M (top) and CPMV-T (bottom) to show particle distribution. Micrographs were imaged using a Titan Krios electron microscope and detected using a Falcon II direct electron detector (FEI). Scale bar in 50 nm. (b) EM density map of CPMV-M (CPMV particle containing RNA-2) determined by cryo-EM to a global resolution of 3.94 Å (EMD-3565). The Large (L) subunit is displayed in green and the Small (S) subunit shown in blue. The bottom panel shows an example of the EM density for an individual β strand from each the L and S subunits displayed as mesh representation. The atomic model within was refined against the EM density. (c) EM density map of the naturally occurring empty CPMV particles known as CPMV-T to 4.25 Å (EMD-3562). The view is identical to that shown in (b).