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. 2017 Apr 3;7:539. doi: 10.1038/s41598-017-00533-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome organisation of CPMV-M containing RNA-2. (a) A 40 Å thick central slab through the unsharpened CPMV-M EM map (at 4.25 Å resolution; also deposited with the EMD-3565 deposition. Suggested contour level is 0.012). A view down the two-fold axis is shown. The coat proteins are coloured as before with the extra EM density attributed to RNA coloured pink. (b) A view beneath the capsid at the two-fold axis showing the strongest density for RNA. (c) Zoomed in view to highlight RNA-protein interactions. The strongest density bridges between the capsid and RNA are at amino acids Asn174 and Arg17 from the L subunit. Trp190 binds strongly to RNA in CPMV-B virions (containing RNA-1), here we can see no density between Trp190 and the EM density attributed to RNA-2.