Table 1.
Microscopy and image processing details for CPMV-M and CPMV-T.
| CPMV-M | CPMV-T | |
|---|---|---|
| Data Collection | ||
| Particles in final reconstruction | 10,850 | 4,696 |
| Pixel size (Å/pixel) | 1.10 | 1.10 |
| Defocus range (μm) | 0.35–10.0 | 0.35–8.0 |
| Voltage (kV) | 300 | 300 |
| Dose (e−/Å2) | 42 | 42 |
| Particle Numbers | ||
| Autopicking | 24,976 | 5,594 |
| Particle sorting | 21,931 | 5,457 |
| 2D classification | 15,228 | 5,165 |
| 3D classification | 10,850 | 4,696 |
| EM Refinement | ||
| Final resolution (Å) | 3.94 | 4.25 |
| Experimental B-factor (Å2) | −144.1 | −185.8 |
| EMDB accession number | EMD-3565 | EMD-3562 |
| Model Validation | ||
| MolProbity score | 1.41 (97th percentile) | 1.32 (98th percentile) |
| Clashscore (all atoms) | 1.77 (99th percentile) | 1.51 (99th percentile) |
| Ramachandran favoured | 92.34% | 93.45% |
| PDB ID | 5MSH | 5MS1 |