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. 2017 Apr 3;7:567. doi: 10.1038/s41598-017-00505-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL1β is negatively correlated with testosterone and the number of macrophages in testes of mice-TIS and mice-TMS. (A) Serum and testis IL1β (ELISA) in G4H and G8C4H groups compared with their control group. (B) Correlation analysis demonstrated that both serum (left) and testis (right) IL1β levels were negatively correlated with testosterone in mice. (C) Immunohistochemical detection of the macrophage-specific antigen F4/80 (black arrows) in testes from each group of mice and comparison of the F4/80-positive cell ratio between G4H and G4C, G8C4H and G12C mice. Scale bars = 100 μm.