Figure 3. An Individual-Based Model to Predict Population Growth Rate.

(A) All cells are born as daughter cells (originating from the dashed arrows) and become mothers after completion of their first bud. At birth or after the completion of a bud, each cell is assigned a new DT that is dependent on the DT variability and epigenetic DT behaviors (Model_Stoch,epig). The model can also be run without taking into account epigenetic behaviors (Model_{Stoch,no-epig}) or stochasticity (Model_Determ). See also the Results section.

(B) Modeled population growth rates (Modeled growth rate_{Stoch,no-epig}) versus growth rates measured on solid media (microcolony growth rates). Microcolony growth rates were measured by microscopically tracking the cell count increase of microcolonies over time.

(C) Modeled population growth rates (Modeled growth rate_{Stoch,no-epig}) versus growth rates measured in liquid cultures.

Error bars represent bootstrapped SDs. mDT₁, the first doubling time of the mother cell; mDT₂, the second doubling time of the mother cell; mDT_1/2, the first or second doubling time of the mother cell; dDT_1/2, the doubling time of the first or second daughter cell. See also Supplemental Experimental Procedures and Figures S2, S3, and S6.