Figure 3.

Expression levels of ORMDL3 protein in lung tissues of the (A) asthmatic model (B) budesonide-treated and (C) healthy control groups was investigated using immunohistochemistry. Magnification, ×100. ORMDL3 protein expression was quantified using (D) ImageJ analysis of stained sections and (E) western blotting. (F) mRNA expression level of ORMDL3 in lung tissues was determined using reverse transcription-quantitative polymerase chain reaction. (G) ORMDL3 protein expression was positively correlated with bronchial wall thickness, r=0.968, P<0.01. *P<0.05 and **P<0.01 vs. control; ^#P<0.05 and ^##P<0.01 vs. treated. ORMDL3, orosomucoid-like 3.