Figure 6.

Experimental procedure. Eight-week-old female BALB/c mice were randomly divided into three groups (n = 36 per group); groups 1 and 2 were orally intubated with 25% trehalose or LG2055 (1 × 10⁹ CFU/200 μL/mouse) throughout the experiment once per day for 5 weeks. After administration for 3 weeks, mice were orally infected with P. gingivalis (1 × 10⁸ CFU/100 μL with 5% CMC/mouse) once per day for 2 weeks. The third group received 5% CMC without oral intubation. Mice were sacrificed 0, 1, 2, and 3 weeks (n = 5 at each time point) after LG2055 administration for a mouse β-defensin-3 assay, and were sacrificed 1 and 30 days (n = 8 at each time point) after P. gingivalis infection for mouse β-defensin-3 assay, GMC cultures, bacterial detection, histological analysis, and alveolar bone loss evaluation. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/bnmrY9.