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. 2017 Apr 6;7:698. doi: 10.1038/s41598-017-00652-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure. 3 — Cytokine levels in the hypothalamus. Bars represent mean cytokine level ± SEM. Data are presented as pg cytokine/mg protein with statistical analysis conducted on Blom transformed (normalized) data. The presence of an asterisk (*) indicates a main effect of AA severity, with the comparison to the control group, unless otherwise indicated. The “×” symbol indicates a significant main effect of colony. The “§” symbol indicates a significant colony × AA severity interaction, with the symbol denoting a comparison between colonies, within rats of the same AA severity state. Percentages on bars indicate the percent of rats with detectable cytokine levels, (within each AA severity state) and these data (a, b, d) were not analyzed statically due to non-normal distribution. Post hoc: *^/§/× p < 0.05; **^/§§ p < 0.01; ***p < 0.001. Control: saline-injected; Adj/NA: no clinical signs of AA following CFA injection; Adj/M-M: mild-moderate AA, clinical score ≥1, <8; Adj/S: severe AA, clinical score ≥8.