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. 2017 Mar 31;7:514. doi: 10.1038/s41598-017-00643-5

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunofluorescent localisation of Eg-DHFR in different stages of E. granulosus. Eg-DHFR was localised in the protoscolex, germinal layer and adult worm using specific anti-rEg-DHFR IgG (positive), or preimmune serum (negative). The nucleus DNA was stained with DAPI (blue). Abbreviations: Teg, tegument; PR, parenchymal region; GL, germinal layer; LL, laminated layer. Scale bars: 1 mm.