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. 2017 Mar 31;7:514. doi: 10.1038/s41598-017-00643-5

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Enzymatic activity of rEg-DHFR and inhibitory effect of antifolates. (A) The kinetic parameters were calculated by using a Michaelis-Menten curve fit using GraphPad Prism® software (nonlinear regression). (B) Inhibition curves of rEgDHFR and human DHFR enzyme activity by methotrexate. (C) Inhibition curves of rEgDHFR and human DHFR enzyme activity by aminopterin. (D) Inhibition curves of rEgDHFR and human DHFR enzyme activity by trimethoprim. (E) Inhibition curves of rEgDHFR and human DHFR enzyme activity by pyrimethamine. The IC₅₀ of antifolates were calculated and represented using means standard errors of the means. Chemical structures of the antifolate drugs methotrexate (i), trimethoprim (ii), aminopterin (iii), and pyrimethamine (iv).