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. 2017 Mar 31;7:514. doi: 10.1038/s41598-017-00643-5

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The protoscolicidal activity of antifolates in vitro. Vitality of protoscoleces (PSCs) following in vitro treatment with different concentrations of antifolates. Statistically significant differences were compared by analysis of variance (ANOVA). A value of P < 0.05 was considered statistically significant. (A) Praziquantel, ANOVA: F _{(3, 24)} = 10.87, P < 0.0001. (B) Methotrexate, ANOVA: F _{(3, 28)} = 0.3907, P = 0.7606. (C) Aminopterin, ANOVA: F _{(3, 28)} = 0.4456, P = 0.7223. (D) Pyrimethamine, ANOVA: F _{(3, 28)} = 4.431, P = 0.0114. (E) Trimethoprim, ANOVA: F _{(3, 28)} = 4.444, P = 0.113. Data shown are mean values from three experiments.