Indirect ELISAs (iELISAs) for the detection of cystic echinococcosis in sheep. (A) The rEg-DHFR iELISA. Statistically significant differences between E. granulosus-positive serum and the other positive sera were tested by one-way ANOVA using GraphPad Prism software (ANOVA: F (2, 35) = 17.21, P < 0.0001). A statistically significant difference between the E. granulosus-positive group and the negative group was determined by t-test (t-test: t (70) = 12.04, P < 0.0001). P values < 0.05 were considered significant. (B) The hydatid fluid iELISA. ANOVA: F (2, 35) = 0.8212, P = 0.4482; t-test: t (70) = 4.708, P < 0.0001. (C) The rEg-cystatin iELISA. ANOVA: F (2, 35) = 3.019, P = 0.0617; t-test: t (70) = 6.765, P < 0.0001. (D) The rEg-annexin iELISA. ANOVA: F (2, 35) = 1.324, P = 0.2791; t-test: t (70) = 7.411, P < 0.0001. (E) The rEg-tetraspanin-1 iELISA. ANOVA: F (2, 35) = 0.4218, P = 0.6592; t-test: t (70) = 5.894, P < 0.0001. (F) The rEg-prohibitin iELISA. ANOVA: F (2, 35) = 1.684, P = 0.2003; t-test: t (70) = 3.078, P = 0.003. (G) The rEg- glutaredoxin-1 iELISA (Our previous published data, ref. 35.). ANOVA: F (2, 18) = 28.41, P < 0.0001; t-test: t (46) = 15.12, P < 0.0001.