Table 2.
Comparative evaluation of the serological assays for diagnosis of CE in sheep.
| ELISAs | Optimal concentration of antigens | Optimal serum dilution | Cut-off values | Analytical specificity | Analytical sensitivity | Diagnostic specificity (DSP) | Diagnostic sensitivity (DSN) | Diagnostic accuracy |
|---|---|---|---|---|---|---|---|---|
| DHFR | 0.6 μg/well | 1:320 | 0.4014 | 85.7% (12/14) | 1:6400 | 89.58% (43/48) | 95.83% (23/24) | 91.67% (66/72) |
| Tetraspanin-1 | 0.4 μg/well | 1:640 | 0.6132 | 14.29% (2/14) | 1:3200 | 43.75% (21/48) | 75% (18/24) | 54.17% (39/72) |
| Prohibitin | 1.2 μg/well | 1:80 | 0.6397 | 57.14% (8/14) | 1:1600 | 66.67% (32/48) | 50% (12/24) | 61.11% (44/72) |
| Annexin | 0.4 μg/well | 1:640 | 0.5369 | 21.43% (3/14) | 1:6400 | 83.33% (40/48) | 87.5% (21/24) | 84.72% (61/72) |
| Cystatin | 0.8 μg/well | 1:320 | 0.427 | 50% (7/14) | 1:1600 | 77.08% (37/48) | 79.17% (19/24) | 77.78% (56/72) |
| Glutaredoxin-1* | 1.6 μg/well | 1:320 | 0.481 | 64.3% (9/14) | 1:3200 | 100% (24/24) | 95.83% (23/24) | 97.9% (47/48) |
| Hydatid fluid | 1:1600 | 1:160 | 0.467 | 71.43% (10/14) | 1:1600 | 81.25% (39/48) | 66.67% (16/24) | 76.39% (55/72) |
*Our previous published data, ref. 35.