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. 2017 Mar 30;7:509. doi: 10.1038/s41598-017-00626-6

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© The Author(s) 2017

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β-elemene inhibited growth and induced cell cycle arrest in NPC cells. (A,B) C666-1 (A) and HNE2 (B) cells were treated with increased concentrations of β-elemene for up to 48 h or to β-elemene (20 μg/mL) for up to 72 h. (C) HNE2 cells were treated with indicated concentrations of β-elemene for 24 h. The cells were collected and processed for analysis of cell cycle distribution. Cell cycle was analyzed by flow cytometry after propidium iodide (PI) staining, and the percentages of the cell population in each phase (G0/G1, S and G2/M) of cell cycle were analyzed by Multicycle AV DNA Analysis Software. Data are expressed as a percentage of total cells. Values are given as the mean ± SD, from 3 independent experiments performed in triplicate. *Indicates significant difference as compared to the untreated control group (P < 0.05).