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. 2017 Apr 3;7:530. doi: 10.1038/s41598-017-00696-6

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Dose dependent cytotoxicity study results of GAR, blank-NPS and GAR-NPs against different cancer cell lines, (b) IC₅₀ values of GAR and GAR-NPs obtained during MTT assay at 24 h and 48 h. Flow cytometer analysis of cell apoptosis using annexinV-FITC and PI staining in (c) B16F10 melanoma cells and (e) KB cells. DAPI staining showing bright fluorescence due to condensed chromatin (apoptotic condition) in (d) B16F10 melanoma and (e) KB cells.