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. 2017 Mar 28;7:544. doi: 10.1038/s41598-017-00657-z

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Scheme of the studied proteins (α-synuclein and TDP-43) with their fused tags (HA and GFP). (B) SH-SY5Y cells stably expressing tagged TDP-43 and α-synuclein. I–III Over-expressed HA-tagged α-synuclein is located in the cytoplasm. IV–VI Over-expressed HA-tagged TDP-43 is located in the nucleus. VII–IX Over-expressed GFP-tagged α-synuclein is located in the cytoplasm. Cells were fixed and stained with DAPI or immuno-stained with a specific HA-antibody and viewed using confocal microscopy. Scale bar: 50 µm.