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. 2017 Mar 28;7:544. doi: 10.1038/s41598-017-00657-z

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Three-dimensional localization of HA-tagged α-synuclein in GFP-expressing ‘recipient’ cells. GFP-expressing cells were co-cultured with ‘donor’ cells expressing HA-tagged α-synuclein for three days and 3D volume reconstruction of the Z-series (A) with XZ-slice projection (B) was performed. DAPI (blue), anti-HA-antibody (red). The interval between individual Z-stack serial images is 0.3 µm.