Fig. 5.

Simultaneous voltage imaging and scanless glutamate uncaging from individual spines using two independently controlled and synchronized CGH patterns of illumination. (a) Fluorescence image of a section of basal dendrite under wide-field illumination used to generate illumination pattern for voltage imaging (green) and glutamate uncaging (blue). (b) The same dendritic section under patterned illumination. (c) Optical signals corresponding to a subthreshold signal evoked by glutamate uncaging onto one spine and suprathreshold (bAP) response evoked by depolarizing current pulse. Signals from spine head (red) and from parent dendrite (black) are shown. (d) Comparison of subthreshold signals from the dendritic region at the base of the spine evoked by uncaging glutamate onto one spine and two spines simultaneously. (e) Signals from spine head (red) and parent dendrite (black) evoked by simultaneous uncaging of glutamate onto two spines. (f) Patch electrode somatic recordings of the subthreshold responses related to glutamate uncaging onto one and two spines.