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. 2017 Apr 5;7:649. doi: 10.1038/s41598-017-00640-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of TWIK2 in lysosomal membranes. Representative images of MDCK cells expressing (A) GFP-coupled compartment markers and rat TWIK2HA, and (B) Lamp1-GFP together with mouse, rat or human TWIK2HA. TWIK2 was stained with HA antibody (red) and nuclei were visualized with Hoechst33342 (blue). Scale bar: 10 µm. (C) Image of a living MDCK cell expressing rat TWIK2-GFP and incubated with lysotracker (red), staining the lysosomal lumen. Scale bar: 10 µm. (D) Electronic microscopy images of a cell expressing rat TWIK2 (lower panel) or of a control cell (upper panel). Scale bar: 2 µm. Lysosomes are dense bodies (plain arrow).