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. 2017 Apr 4;7:619. doi: 10.1038/s41598-017-00682-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ts65Dn mice show abnormal general behavior. (A,B) Male Ts65Dn and the control mice were tested with elevated plus maze. The amount of time (A) spent in the open arm and number (B) of open arm (OA) entrances were analyzed. (C–E) Male Ts65Dn and the control mice were subjected for open field test. Enters into the center area (C), time amount spent in center area (D), and total distance covered (E) were analyzed. (F) Male Ts65Dn and control mice were analyzed locomotor activity by rotarod test. The data are presented as mean ± SEM and statistically analyzed with student t test (A–D) and with two way ANOVA followed by Bonferroni post-test (E,F) (n = 11–12). *p < 0.05; ***p < 0.001.