Table 1.

Summary of parameters acquired for single cells cultured in the absence and presence of Cell Tracer.

Parameter and notation		No Cell Tracer	With Cell Tracer
Number of cells	N	26	22
Migration speed (μm/hr)	v	${16.25}_{-3.9}^{+8.4}$	${11.51}_{-3.8}^{+3.3}$
Step length in x (μm)	l x	${2.5}_{-1.3}^{+2.5}$	${1.9}_{-0.9}^{+1.0}$
Step length in y (μm)	l y	${2.5}_{-1.3}^{+2.5}$	${1.9}_{-0.9}^{+1.0}$
Correlation time (hr)	τ	${0.6}_{-0.4}^{+0.9}$	${0.7}_{-0.5}^{+1.1}$
Diffusivity (μm2/hr)	D	79.8 ± 5.2	49.1 ± 3.5
Time to first division (hr)	t div	${4.9}_{-1.6}^{+5.1}$	${9.3}_{-3.4}^{+3.3}$
Death fraction at 10 hours, %	—	0	23
Death fraction at 20 hours, %	—	4	23

The entries are representative median values, with errors given by the lower and upper quartiles. The exception is the diffusivity, which is presented as a mean and 95% confidence interval. The step lengths and migration speed were calculated by averaging over all cells at all times (15 min intervals). The diffusivity was obtained using the fits to $\overline{L^2}$ shown in Fig. 3 and the correlation time from τ = 2D/v ² for instantaneous speeds.