Figure 3.
Effect of LDLR overexpression on LDL-mediated drug transport in vitro. (A) Hepa1-6 cells were infected with green fluorescent protein (GFP)-tagged mouse LDLR-expressing adenovirus (Ad-LDLR). Two days after infection, LDLR-GFP overexpression was confirmed by western blotting. GFP-expressing adenovirus (Mock) was used as a control. (B) The uptake of LDL solution containing [3H]cholesterol in FBS-free transport buffer was measured in Mock and Ad-LDLR cells. (C–E) In vitro uptake experiments for LDL-associated (C) clopidogrel, (D) ticlopidine, and (E) ticagrelor in Mock and Ad-LDLR cells. Bars represent the mean ± s.e.m. *p < 0.05, **p < 0.01.