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. 2017 Mar 9;15(5):2473–2480. doi: 10.3892/mmr.2017.6308

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Expression levels of miR-133a and osteoblastic markers following osteogenic induction of miR-133a-overexpressing human bone marrow stromal cells in the presence or absence of VK₂. Expression levels of (A) miR-133a, (B) ALP, (C) OCN and (D) Runx2 were determined by reverse transcription-quantitative polymerase chain reaction. (E) ALP activity in cell lysates as determined by an ALP activity assay. (F) OCN concentrations in supernatant as determined by ELISA. (G) ALP expression, as determined by staining. (H) Runx2 protein expression levels were determined by western blotting and (I) quantified by densitometry. Data are expressed as the mean ± standard deviation (n=3). *P<0.05 vs. miR-133a-control. miR, microRNA; VK₂, vitamin K₂; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin.