Figure 4.

CREB-mediated parkin expression induced by hydrocortisone. (A) Expression of parkin and CREB in SH-SY5Y cells in response to treatment of hydrocortisone in increasing dose determined by western blot. (B) Relative levels of parkin or CREB were normalized to that of β-actin (n = 3). (C) Western blot analysis of parkin and CREB expression in SH-SY5Y cells transiently transfected with gRNA specific for CREB or control gRNA followed by hydrocortisone treatment. (D) Relative parkin or CREB levels were normalized to that of β-actin in the indicated experimental groups (n = 3). (E) Western blot analysis of parkin and CREB levels in SH-SY5Y cells. Cells were treated with glucocorticoid receptor antagonist RU-488 followed by hydrocortisone treatment to examine parkin and CREB induction. (F) Relative parkin and CREB induction in response to hydrocortisone treatment in SH-SY5Y cells pretreated with RU-486 (mifepristone) or vehicle control (n = 3 per group). Quantified data are expressed as mean ± s.e.m. **P < 0.01 and ***P < 0.001, ANOVA test followed by Tukey post hoc analysis (B,F, and parkin quantification in Fig. 4D) or nonparametric Kruskal-Wallis ANOVA test (CREB quantification in Fig. 4D). Full blots (for cropped images in A,C,E) are presented in Figure S6.