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. 2017 Apr 4;7:631. doi: 10.1038/s41598-017-00632-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy showing the mitochondrial localization of yeast Lon protease. (A) The mitochondrial network in Saccharomyces cerevisiae cells after DiOC₆ staining. (B) The localization of Lon-GFP expressed from pUG35 in S. cerevisiae. (C) Visualization of S. cerevisiae nuclear (large red dots) and mitochondrial DNA (smaller red spots) after DAPI staining. The blue colour of DAPI was changed to red in the imaging software. (D) The merged signal confirming the co-localization of Lon-GFP and mtDNA. (E) A negative control, showing the non-specific GFP signal from an empty pUG35 plasmid which localizes to the cytosol of S. cerevisiae cells.