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. 2017 Apr 4;7:631. doi: 10.1038/s41598-017-00632-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EMSA of Abf2 with various DNA substrates. Increasing concentrations of Abf2 (0, 50, 100, 200 and 500 nM) were incubated with 75 nM fluorescently labelled DNA probes, whose structures are shown schematically above the corresponding lines. The asterisk (*) indicates the position of the Cy3 fluorescent dye. Samples were separated in 8% native polyacrylamide gels in 0.5 × TBE.