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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Anal Chem. 2016 Aug 29;88(18):9029–9036. doi: 10.1021/acs.analchem.6b01653

Figure 4. Fluorescence monitoring of Zn2+ diffusion in HEK 293T cells.

Figure 4

(A) Fluorescence and brightfield images of cells expressing ZnGreen1 at cell surface and ZnRed in the nucleus before and after addition of Zn2+/pyrithione (scale bar: 20 μm). (B) Represented traces (n = 6 cells) for intensity changes, showing Zn2+-induced decrease of ZnGreen1 fluorescence (extracellular) and increase of ZnRed fluorescence (nuclear).