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. 2017 Apr 3;37(5):2633–2651. doi: 10.3892/or.2017.5547

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Copyright: © Arriaga et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Labile zinc induction upon differentiation. (A) Butyrate induces intracellular labile zinc levels in HT-29 cells, as measured by fluorimetry using the Fluozin-3AM (FZ) probe. (B and C) Both CDX2 and HSI induction by butyrate are blunted by TPEN pre-treatment, whereas there is no effect on (D) TFF3 expression. (E and F) Labile zinc levels are also increased in the post-confluency differentiation model of HT-29 cells, as measured by (E) epifluorescence microscopy (F) and fluorimetry. (G) Daily doses of TPEN stimulate TFF3 expression of post-confluent cultures. AFC, average fold-change; *p<0.05, **p<0.01 and ***p<0.001.