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. 2017 Mar 17;37(5):2611–2619. doi: 10.3892/or.2017.5514

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression of hypoxia-inducible factor 1α (HIF-1α) is upregulated by lipopolysacharide (LPS) but downregulated by propofol in NSCLC cells. (A) A549 cells were exposed to different concentrations of LPS for 12 h. The expression levels of HIF-1α protein were then analyzed by western blotting. GAPDH served as a control. (B) Quantitative data of western blotting in A; *P<0.05. (C) Following treatment as described in A, the levels of HIF-1α mRNA were analyzed by qRT-PCR. The mRNA levels of the indicated genes were normalized to actin; *P<0.05. (D) Following treatment as described in A, generation of ROS was measured as outlined in Materials and methods; *P<0.05. (E) A549 cells were co-treated with 10 µg/ml LPS and different concentrations of propofol for 12 h. The expression levels of HIF-1α protein were then analyzed by western blotting. GAPDH served as a control. (F) Quantitative data of the western blotting in E; *P<0.05. (G) Following treatment as described in E, the levels of HIF-1α mRNA were analyzed by qRT-PCR. The mRNA levels of the indicated genes were normalized to actin; *P<0.05. (H) Following treatment as described in E, generation of ROS was measured as outlined in Materials and methods; *P<0.05.