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. 2017 May 12;13(5):e1006757. doi: 10.1371/journal.pgen.1006757

Fig 3. Brg1 cooperates with maternal Wnt signaling for axis formation in X. laevis.

Fig 3

Panels (A-C) axial rescue of DMZ-injected BMO1 morphants (10ng) by coinjected ß-catenin mRNA (250pg). (A) DMZ-injected BMO1 morphant with severly impaired antero-posterior axis (n = 64/75 “headless”); (B) overexpression of ß-catenin in DMZ does not cause gross abnormalities (n = 26/32 wt-like); (C) coinjection of BMO1 and ß-catenin rescues the truncated 1° axis (n = 12/21 heads with eyes and cement gland). Panels (E-G) show that de novo induction of a second embryonic axis by ß-Catenin (250pg) depends on endogenous Brg1 activity. (E) VMZ-injected BMO1 morphants (10ng) display wt-like morphology (n = 12/14; see also Fig 2D); (F) VMZ-injection of 250pg β-catenin mRNA induce a second body axis (n = 38/42 complete secondary heads with eyes and cement gland); (G) Coinjection of BMO1 with β-catenin mRNA blocked almost completely the induction of secondary axes (n = 18/20 single axis status; 1 double axis remaining). Correct targeting of injections was verified by fluorescence from coinjected GFP mRNA (100pg; see inserted images).