Skip to main content
. 2017 May 12;13(5):e1006757. doi: 10.1371/journal.pgen.1006757

Fig 4. Brg1 protein knockdown diminishes gene expression in the BCNE center.

Fig 4

Pictures show dorsal views of whole mount RNA in situ hybridization (WMISH) at late blastulae (NF9, X. laevis) with gene expression patterns representative for the experimental condition. Injection conditions are indicated on top: CoMO (60ng/embryo), BMO1 (60ng/embryo), Rescue (BMO1 plus hBRG1 mRNA [1ng/embryo]). Reagents were radially injected into the animal hemisphere. The mRNA expression patterns of chordin, noggin and nodal-related 3 (nr3) in control morphants (panels A-C) were reduced in BMO1 morphants (panels A’–C’), but restored by coinjection of human brg1 mRNA (panels A” –C”). The graphs (A”‘–C”‘) depict phenotypic penetrance (n = 3–4 experiments/condition). The expression of siamois was not affected by BMO1 (panels D + D’, n = 2 experiments/gene).