Fig 5. Reduced Brg1 protein levels impair organizer function.
X. laevis embryos were radially injected with 40ng of indicated MOs and fixed at early. Representative images of the predominant morphant expression patterns are shown by WMISH in vegetal (A, B, G-L) or dorsal (D, E, N, O) side views. The Organizer region is represented by nr3 (A-C), otx2 (D-F) and foxA4 (G-I). Inserts depict deep staining in bisected embryos for nr3 or blastopore lip staining for otx2. Non-organizer mesoderm is represented by the BMP target genes vent1 (K M) and vent2 (N-P). Inserts show side views for vent1 and vegetal views for vent2 (n = 2-4 independent experiments/gene).