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. 2017 Apr 11;37(5):2652–2662. doi: 10.3892/or.2017.5573

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Copyright: © Ouyang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Western blotting, qPCR, and IHC determination of GINS2 mRNA and protein expression. (A) Western blotting and qPCR of GINS2 expression in normal cervical tissues and cervical cancer cell lines. (B) Western blotting of GINS2 expression in 8 pairs of matched cervical cancer (T) and adjacent non-cancerous cervical tissues (ANT) and average T/ANT ratios of GINS2 mRNA expression quantified by qPCR in 8 pairs of matched cervical cancer tissues. Expression levels were normalized to α-tubulin expression. Error bars represent standard deviation (SD) calculated from 3 parallel experiments. *P<0.05. (C) IHC assay of GINS2 protein expression in 8 pairs of matched cervical cancer tissues.