Figure 1.

Expression levels of smurf2, SnoN and p-Smad2 in NRK-52E cells incubated with low glucose medium. (A) Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect the mRNA levels of smurf2, SnoN and Smad2 in NRK-52E cells incubated with low glucose medium at different time points. (B) Western blot analysis was performed to detect the expression levels of smurf2, SnoN, Smad2 and p-Smad2 in NRK-52E cells incubated with low glucose medium at different time points. *P<0.05 and ***P<0.001, vs. control. SnoN, ski-related novel protein N; p-Smad2, phosphorylated small mothers against decapentaplegic; smurf2, Smad ubiquitination regulatory factor 2.