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. 2017 Mar 9;15(5):2560–2566. doi: 10.3892/mmr.2017.6307

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression levels of SnoN and p-Smad2 in NRK-52E cells transfected with recombinant adenovirus. (A) Immunofluorescence analysis of the expression of smurf2 in NRK-52E cells infected with AD-smurf2. (B) RT-qPCR analysis was performed to detect the mRNA levels of smurf2, SnoN and Smad2, and western blot analysis was performed to detect the expression levels of smurf2, SnoN and p-Smad2 in NRK-52E cells infected with AD-smurf2. (C) Immunofluorescence analysis of the expression of smurf2 in NRK-52E cells treated with AD-shsmurf2. (D) RT-qPCR analysis was performed to detect the mRNA levels of smurf2, SnoN and Smad2, and western blot analysis was performed to detect the expression levels of smurf2, SnoN and p-Smad2 in NRK-52E cells infected with AD-shsmurf2. ***P<0.001, vs. control. SnoN, ski-related novel protein N; p-Smad2, phosphorylated small mothers against decapentaplegic; smurf2, Smad ubiquitination regulatory factor 2; AD, adenovirus; NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.