Figure 4.

Effects of cholesterol on the expression of heme oxygenase-1 (HO-1)-related molecules. (A) Western blot analysis was used to evaluate the expression levels of nuclear factor erythroid 2-like 2 (Nrf2), AKT, p-AKT, mTOR and p-mTOR proteins (B–D) The expression levels of Nrf2, AKT, p-AKT, mTOR and p-mTOR proteins as quantified by densitometry. β-actin was used as an internal control. Data are presented as means ± SEM of three individual experiments. P-values were calculated using one-way ANOVA. ^*P<0.05 vs. the control group.