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. 2017 Apr 28;13(4):e1006762. doi: 10.1371/journal.pgen.1006762

Fig 6. WDR23 restores NRF2 regulation in cancer cells.

Fig 6

(A,B) Overexpression of GFP:WDR23 isoform 1 (A) or GFP:WDR23 isoform 2 (B) can deplete activated endogenous NRF2 (Alexa Fluor 594, red) from the nucleus (DAPI, blue) in A549 lung cancer cells. Scale bar, 20μm. Arrows denote transfected cells. (C) Quantification of nuclear or diffuse localization of endogenous NRF2 in cells over expressing GFP:WDR23 isoform 1 (n = 74), GFP:WDR23 isoform 2 (n = 84) or non-transfected cells from the same experiment (n = 490). Each bar represents an independent immunostaining experiment. Fisher’s exact test; **P<0.01, ***P<0.001. (D,E) A549 cancer cells overexpressing WDR23 have reduced levels of the NRF2 target genes GSTA1 (Control n = 12, Iso 1 n = 6, Iso 2 n = 6) (D) and PRDX1 (Control n = 12, Iso 1 n = 6, Iso 2 n = 6) (E). Data are mean ± s.e.m.; one-tailed t-test relative to control samples. *P<0.05.