Fig 1. Small indels around rs3812049 reduce expression of both SLC12A2 and LINC01184 in HUDEP-2 cells.

HUDEP-2 human erythroid precursor cells were transduced with lentivirus expressing Cas9 and a guide RNA, either nontargeting (NT) or targeting cleavage at rs3812049, and selected with antibiotics. Seven days after transduction, expression of SLC12A2 and LINC01184 in the population of edited cells was measured by quantitative reverse transcription PCR. Experiment was performed in biologic triplicate. Bars indicate means and error bars indicate standard deviation. T-tests showed significant differences in expression of both SLC12A and LINC01184 upon introduction of indels around rs3812049 (p < 0.01 for each comparison to unedited controls).