. 2017 Jan 13;68(8):1955–1972. doi: 10.1093/jxb/erw460

Table 1.

Sample description and numbers of RNA-Seq reads for samples

Developmental stage		Sampled tissues	Genotype^*	Number of reads**
Developmental stage		Sampled tissues	Genotype^*	Rep 1	Rep 2	Rep 3
S1 3–5 DAF ^***	Seed set: Acellular endosperm in seed, undergoing active cell division in embryo and maternal seed coat and seed pod.	Seed pod with whole seed	V1	77.1 M	64.6 M	63.1 M
S1 3–5 DAF ^***		Seed pod with whole seed	V2	77.1 M	64.6 M	63.1 M
S2 10–14 DAF	Seed growth: Endosperm is degenerated and its cellular contents are assimilated by the cotyledons.	Whole seed	V1	66.4 M	64.6 M	76.2 M
S2 10–14 DAF		Whole seed	V2	63.3 M	68.2 M	65.2 M
S3-1 20–22 DAF	Seed filling: Seed coat and cotyledon are separated from whole seed with 90–100mg fresh weight (around one-third of final weight); accumulation of nutrients, oil, storage proteins in cotyledon.	Seed coat	V1	73.1 M	71.3 M	77.1 M
S3-1 20–22 DAF		Seed coat	V2	74.3 M	65.7 M	85.6 M
S3-2 20–22 DAF		Seed cotyledon	V1	70.5 M	69.4 M	74.2 M
S3-2 20–22 DAF		Seed cotyledon	V2	60.3 M	61.9 M	71.4 M

* Genotype V1 and V2 are the large and small seed cultivar, respectively; ** number of processed clean reads obtained from three biological replicates of RNA high-throughput sequencing [expressed in millions (M)]. DAF, days after fertilization.