Table 2.
Total sugars in developing pods (~10–15mm long with presence of small immature seed/s), second youngest fully-expanded leaves (petiole+leaflets), stems and roots of chickpea (Rupali) subjected to control or 30mM NaCl treatments either without infusion or with stem-infusion with water or 0.44M sucrose (Ψπ=–1.1MPa)
Plants were grown in nutrient solution culture and NaCl treatments were imposed on 20-day-old plants for 97 d (20/15±2 °C day/night air temperatures). Infusion treatments were started at the time of flowering (42-day-old plants) and continued until maturity. Tissues were harvested after 28 d of infusion treatments. Values are means±SE (n=4). Two-way ANOVA was used to compare salt (S), infusion (I) and salt × infusion interaction (S × I) effects (***, significant with P<0.001; **, significant with P<0.01; *, significant with P<0.05; n.s., not significant with P>0.05). Different upper case superscript letters for means within a column or across a row indicate significant differences between means for salt treatments or infusion treatments at P=0.05, respectively, whereas different lower case superscript letters indicate significant differences for salt × infusion interaction at P=0.05.
| Tissue | Treatment | Total sugars (µmol hexose equivalents g–1 EtOH insoluble dry mass) |
||||
|---|---|---|---|---|---|---|
| No Infusion |
Water Infusion | Sucrose infusion | Mean | Significance | ||
| Developing pods | Non-saline | 28.9a±2.0 | 28.7a±0.7 | 29.2a±1.6 | 28.9A | salt** |
| 30mM NaCl | 22.5b±1.7 | 23.5b±1.4 | 30.3a±2.0 | 25.5B | infusion* | |
| Means | 25.7B | 26.1B | 29.7A | salt × infusion* | ||
| Leaves | Non-saline | 33.5±2.8 | 32.9±1.4 | 48.3±3.4 | 38.2A | salt*** |
| 30mM NaCl | 26.3±2.8 | 25.7±1.5 | 37.1±1.7 | 29.7B | infusion*** | |
| Means | 29.9B | 29.3B | 42.7A | salt × infusionn.s. | ||
| Stems | Non-saline | 43.3±1.9 | 46.6±3.0 | 63.1±1.5 | 51.0A | salt*** |
| 30mM NaCl | 22.9±3.1 | 25.4±1.6 | 42.9±5.9 | 30.4B | infusion*** | |
| Means | 33.1B | 36.0B | 53.0A | salt × infusionn.s. | ||
| Roots | Non-saline | 62.0±7.4 | 71.3±11.5 | 75.2±3.6 | 69.5A | salt*** |
| 30mM NaCl | 37.7±2.4 | 32.2±0.6 | 45.7±2.0 | 38.5B | infusion n.s. | |
| Means | 49.9 | 51.7 | 60.5 | salt × infusionn.s. | ||