Video 5. Shape changes, rearrangements and fusion events of L_o domains during actomyosin contraction.

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DOI: 10.7554/eLife.24350.009

The phase-separated and DiD labeled (red) membrane (3:3:1 DOPC:PSM:Cholesterol) containing L_o domains (dark) is shown in the upper channel. The middle channel shows Alexa-488-phalloidin-labeled actin filaments (green) that bind to the L_o domains. The lower channel shows the merge of both channels. TIRFM image sequence was acquired at 5 s. time intervals and contains 500 frames. The video is displayed at 15 frames per second (fps). Total time: 41.6 min. Scale bar, 10 µm. (Compressed JPG avi; 28 MB).

DOI: http://dx.doi.org/10.7554/eLife.24350.009