Video 7. Disappearance of small L_o domains during actomyosin contraction.

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DOI: 10.7554/eLife.24350.011

Small L_o domains often vanish during actomyosin contractions. The phase-separated and DiD-labeled (red) membrane (3:3:1 DOPC:PSM:Cholesterol) containing L_o domains (dark) is shown in the left channel. The middle channel shows Alexa-488-phalloidin-labeled actin filaments (green) that bind to the L_o domains. The right channel shows the merge of both channels. TIRFM image sequence was acquired at 5 s. time intervals and contains 493 frames. The video is displayed at 120 frames per second (fps). Total time: 41 min. Scale bar, 5 µm. Corresponds to Figure 3G. (Compressed JPG avi; 4.1 MB).

DOI: http://dx.doi.org/10.7554/eLife.24350.011