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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Hum Mutat. 2017 Apr 13;38(6):736–744. doi: 10.1002/humu.23218

Figure 2. P2X7 functional assay.

Figure 2

A) ATP induced ethidium uptake was used to measure P2X7 pore formation in HEK293 cells transfected with various P2X7 and/or P2X4 constructs. The mean fluorescent intensity of ethidium uptake into cells was measured at 5 sec intervals by two-color flow-cytometry. B) P2X7 expression on the surface of HEK 293 cells transfected with AcGFP-tagged P2X7 and/or P2X4 constructs. Bars represent mean values and SEM of three replicate experiments. WT = wild type. *p<0.05, **p<0.01, ***p<0.001.