Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Microbiol. 2017 Jan 24;19(6):10.1111/cmi.12719. doi: 10.1111/cmi.12719

Figure 1. RBC contain TRiC subunits in the cytosolic fraction.

Figure 1

A, Lysates from uninfected RBC (U), trophozoite-stage infected RBC (I) or saponin isolated parasites (P) were extracted with RIPA buffer and probed with antibodies against human TRiC subunits i) α, ii) β, iii) δ, iv) ζ and v) θ. PfEF-1α is a cytosolic parasite protein. B, Uninfected (uRBC) or infected (iRBC) RBC samples (T = total sample) were treated with 0.05 % saponin (20 s, room temperature), releasing the soluble contents of the RBC and parasitophorous vacuole (SN = supernatant fraction), leaving the insoluble pellet (P = pellet fraction). Human α/β spectrin is a control for the insoluble RBC fraction, and PfEF-1α is a soluble parasite protein. Equivalent fractions were loaded in all blots. Immunoblots are representative of two-four independent experiments. Marker bands are in kDa.