SIVmac239 (A), SIVmac251 (B) or SHIVSF162P3N (C) were incubated with macaque SEVI (50 μg/mL) for 1 h. The mixture was then further incubated with or without the indicated concentrations of EGCG (10, 25, 50 μM) for 0.5 h prior to infection of TZM-bl cells which were then washed once with PBS 3 h postinfection. At 48 h postinfection, the cells were lysed and the luminance (Infection, RLU/s) was determined by a microplate reader. Data were expressed as mean ± SD of three independent experiments (*P<0.05, **P<0.01, ***P<0.001, compared with macaque SEVI treatment only).