Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Microbiol. 2017 Jan 23;19(6):10.1111/cmi.12718. doi: 10.1111/cmi.12718

Figure 8. Loss of CDC50 influences growth at acidic and alkaline pH, and sensitivity to salt stress.

Figure 8

(A) Tenfold serial dilutions of each strain were spotted onto buffered YPD, and the plates were incubated at 30°C for 2 days before being photographed. (B) Tenfold serial dilutions of the strains were spotted onto solid YPD without or with 1.2 M NaCl, 1.5 M KCl, 1.5 M sorbitol, or 100 mM LiCl. The plates were incubated at 30°C for the following times: sorbitol, 3 days; KCl, 5 days; NaCl, 5 days; LiCl, 10 days. (C) Loss of CDC50 did not influence the localization of RIM101-GFP, but deletion of the genes encoding ESCRT complex proteins or Rim101 regulatory components caused mis-localization. Bar = 10 µm.