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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Neurochem. 2017 May 3;141(5):708–720. doi: 10.1111/jnc.14025

Figure 6.

Figure 6

Dimercaprol protected PC-12 cells from acrolein-mediated membrane damage and cell death based on the Trypan Blue assay. PC-12 cells were exposed to 100 μM acrolein for a total of 4 hrs. Some of the cells were treated with additional different concentrations of dimercaprol after 15 min of acrolein exposure. The viability was determined by the Trypan Blue assay. The cell viability of the control (no acrolein or dimercaprol) was considered 100%. The viability of other groups was expressed as the percentages of the control. The treatment with dimercaprol was similar to the previous LDH assay. Note that dimercaprol reduced acrolein-mediated trypan blue membrane permeability in a dosage-dependent manner. (p<0.05 or p<0.01, ANOVA). All data were expressed as mean ± SEM, n=4.