Table 7.
Summary of different PCR primers available for identification of Baylisascaris species.
| Target gene | Length of the target gene | Species | Primer | Reference |
|---|---|---|---|---|
| ATPase subunit 6 (atp6) | 600bp | B. schroederi | F (5′-CGCGGATCCTTCGATATTCGTGG CCT-3′) R (5′-CGCAAGCTTCTAATATGGTGTCTT CGG-3′) |
Xie et al., 2015 |
| cytochrome oxidase c subunit I (CO1) | 413bp | B. procyonis, B. transfuga, B. columnaris | F: (5′-TTTTTTGGGCATCCTGAGGTTTAT-3′) R: (5′-TAACGACATAACATAATGAAAATG-3′) |
Franssen et al., 2013 |
| cytochrome oxidase c subunit 2 (CO2) | 1578bp | B. schroederi | F (5′-TTTAGAGGTTGGAATGTAGGGT-3′) R (5′- CCATCCCCTTAATCTGCAAT-3′) |
Xie et al., 2015 |
| 483bp | B. procyonis, B. transfuga, B. columnaris | F: (5’-AATTTTAATTGTAGTCTTTTGTTTGG-3′) R: (5’-CTATGATTAGCACCACAAATC-3′) |
Franssen et al., 2013 | |
| mitochondrial cytochrome b cytb | ∼1500bp | B. schroederi | Cytb-1 (5′-GGTGCTATGCTCGGTTACG-3′) Cytb-2 (5′-CCACTAAGACCCTCCATT-3′) |
Zhou et al., 2013 |
| 12s rRNA | 499bp | B. schroederi, B.ailuri, B. transfuga, B. procyonis | F (5′-AGCGGAGGAAAAGAAACTAA3′) R (5′-TGATCCTTCTGCAGG TTCACCTAC-3) |
Li et al., 2012 |
| 18s rDNA | 1708bp | B. schroederi, B. ailuri, B. transfuga, B. procyonis | F (5′-AGCGGAGGAAAAGAAACTAA-3′) R (5′-TGATCCTTCTGCAGG TTCACCTAC-3′) |
Li et al., 2012 |
| 28s rDNA | 751bp | B. schroederi, B. ailuri, B. transfuga, B. procyonis | F (5′-CCCGATTGATTCTGTCGGC-3′) R (5′-TGATCCTTCTGCAGG TTCACCTAC-3′) |
Li et al., 2012 |
| 718bp | B. procyonis, B. transfuga, B. columnaris | F: (5′-CGAGGATTCCCTTAGTAACT-3′) R: (5′-TCGGATAGGTGGTCAACG-3′) |
Franssen et al., 2013 | |
| ITS1-5.8S-ITS2 | 630-650bpa | B. procyonis, B. transfuga, B. columnaris | F: (5’-ATAGTGAGTTGCACACTAATGT-3′) R: (5’-TTATATGCTTAAATTCAGCGGG-3′) ITS2-F: (5′-GCCATTTATGAATTTTCAACATGG-3′) ITS2-R: (5′-AGTTATATGCTTAAATTCAGCGG-3′) |
Franssen et al., 2013 |
| Complete mt genome- (atp6, CO1–CO3, cytb, nad1–nad6 nad4L, 22 transfer RNA (trn) Genes (small (rrnS) and large (rrnL) subunits) |
B. schroederi, B.ailuri, B. transfuga, B. procyonis | See reference for numerous sets of primers. | Xie et al., 2011a, Xie et al., 2011b | |
| ITS2 rDNA | 301 bp | B. transfuga | F (5’-TTATGAATTTTCAACATGGC-3’) R (5’-GTTAGATGCTTAAATTCAGC-3’) |
De Ambrogi et al., 2011 |
| 3′ end of the ITS- 1, complete 5.8S and ITS-2, and the 5′ end of 28S rDNA |
700bp | B. schroederi | zghu (5′- AAGGTGGAGAGAAAGCTCCTC GT-3′) NC2 (5’-TTAGTTTCTTTTCCTCCGCT-3’) |
Zhao et al., 2012 |
| ITSs (18s and 28S) | 1177bp | B. transfuga | F (5′-ACTGCTGTTTCGAGACCTTTCGAG-3′) R(5′-TAGCACCTTCTTTGGACTATAGCC-3′) |
Testini et al., 2011 |
a
Different length in different parasites due to insertions and tandem repeat.