Figure 3.

CRISPR/SpCas9-eloxP-Mediated Site-Specific Gene Editing in 293-AAVS-mTmG Cells

(A) An illustration of the CRISPR/SpCas9-eloxP system that mediated site-specific gene editing in 293-AAVS-mTmG cells. (B) 293-AAVS-mTmG cells were transfected with 2.0 ug plasmids (plasmids coding for Cre or CRISPR/SpCas9-eloxP), and images were obtained at 48 hr post-transfection (red indicates mT; green indicates mG). (C) The level of green fluorescence was quantified. Error bars are SD (n = 3). The p value was calculated by Student’s t test; **p < 0.01. (D) Targeting DNA sequence chromatograms of cells transfected with CRISPR/SpCas9-eloxP are clustered together compared with one loxP remaining in the Cre group. (E) CRISPR/SpCas9-eloxP-mediated NHEJ pattern at the human AAVS1 locus. Pink nucleotides indicate sgRNA. Blue nucleotides indicate indels.