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. 2017 Apr 25;7:378–386. doi: 10.1016/j.omtn.2017.04.018

Figure 4.

Figure 4

CRISPR/SaCas9-eloxP-Mediated Site-Specific Gene Editing in 293-AAVS-mTmG Cells

(A) Targeting sequence and corresponding PAMs for CRISPR/SpCas9 (pX330) and CRISPR/SaCas9 (pX601). (B) Efficiency comparison of CRISPR/SaCas9 and SpCas9-eloxP mediated gene editing. The p value was calculated by Student’s t test; *p < 0.05; **p < 0.01. (C) CRISPR/SaCas9-eloxP-mediated NHEJ pattern at the human AAVS1 locus. Pink nucleotides indicate sgRNA. Blue nucleotides indicate indels. See also Figure S5. (D) CRISPR/SaCas9-eloxP-mediated NHEJ pattern in transient reporter-gene expression assays. Pink nucleotides indicate sgRNA. Blue nucleotides indicate indels.